2.2. Actinomycetes Enrichment
This process was done aseptically. First, the soil slurry
was made by suspending 0.1 g of the collected dry soil in 10
ml distilled water. The slurry was mixed by vortexing for 2
min and four 1 in 10 fold serial dilutions were made from the
slurry. These dilutions were done in duplicates (A and B).
Next, 3 ml volumes of the provided top agar were poured
onto the bottom agar plates. The plates were allowed to set,
after which 0.1 ml portions of each dilution, were then plated
by spreading on the set chitin agar plates. All spread plates
where labelled and incubated at 25 ̊C for a period of 14 days.