Recent years have witnessed significant progresses in engineering of recombinant protein secretion. The relatively simple secretion mechanisms,
Type I and Type V (autotransporters), are increasingly used for secretion of recombinant proteins. The secretion level of target proteins
varied from milligrams to grams per liter. The range of proteins was significantly expanded beyond medical application. Notable additions
include biofuel productions from renewable feedstock. Despite the progress, almost all successes in the engineering efforts come with significant
trials and errors, highlighting the need for a better understanding of secretion systems and rational based methods.
2013 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.