In the present work, a new system has been developed for the rapid bio-anaylsis of genotoxic compounds based on the electrochemical DNA-nano-biosensor. The modification of Au nanoparticles on the electrode surface enhanced DNA immobilization and increased the electrochemical signal. Furthermore, the dsDNA/ Nano/Au biosensor can distinguish different contamination levels of water samples and show good correlations of results with the Comet assay. The analysis of a sample can be performed in minutes instead of days without dependence on cell or bacteria. Therefore, this kind of biosensor could be very useful as an easy and fast screening method for genotoxicity analysis of environment pollution.