The respirometric measurements were carried out to determine the long-term toxicity of the ITE on the C. globosum IMA1. Fig. 3 illustrates the evolution of the specific OUR (Fig. 3a), the biomass growth (Fig. 3b) and the COD (Fig. 3c) of samples taken from a cultivation of the fungus strain on undiluted, twice diluted and four times diluted ITEs corresponding to increased initial concentrations of indigo dye in the ITE. Results showed that C. globosum IMA1 needs about 2 days to adapt to initial culture conditions (Fig. 3a). The specific OUR increased significantly, from the 2nd day to the 5th day, due to the metabolism activities including mycelia growth and degradation of dyes.