Despite this previous research, there is limited information on the mechanism(s) of Au absorption, its chemical behaviour, transport and localisation in plants. Relevant studies suggest that the final site of metal accumulation is the leaf [19] or leaf tip [6] however there are no published reports explaining why or how plants take up Au. Anderson et al. [20] suggested evapotranspiration (the combined effect of evaporation of water from the soil and plant surfaces and the loss of water through stomata in leaves), as a possible mechanism for induced hyperaccumulation in Au. There is a lack of information in the literature on the response of plants at higher Au concentrations and no reports of the extent of uptake of Au in BJ and MS when exposed to Au solutions for extended periods of time. This information is necessary to advance the development ofphytomining and phytoremediation processes for Au.