Objective: To investigate antioxidant potential of methanol extract of Alpinia nigra leaves.
Methods: The study was done by using various in vitro methods such as 1,1-diphenyl-2-
picrylhydrazyl (DPPH), 2,2-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS), nitric
oxide and hydrogen peroxide radical scavenging assays. Phytochemical constituents, total
phenolic content and total flavonoid content of the extract at different concentrations (10-500 µg/
mL) were determined.
Results: Alpinia nigra leaves showed high free radical scavenging activity as evidenced by the
low IC50 values in DPPH (64.51 µg/mL), in ABTS (28.32 µg/mL), in nitric oxide (80.02 µg/mL) and in
H2O2 (77.45 µg/mL) scavenging assays. Furthermore the TPC and TFC of the extract were found to
be 69.25 mg gallic acid equivalent per gram of extract and 78.84 mg quercetin equivalent per gram
of extract respectively.
Conclusions: The results of present comprehensive analysis demonstrated that Alpinia nigra
leaves possess high phenolic, flavonoid contents and potential antioxidant activity, and could be
used as a viable source of natural antioxidants and might be exploited for functional foods and
neutraceutical applications.
Objective: To investigate antioxidant potential of methanol extract of Alpinia nigra leaves.
Methods: The study was done by using various in vitro methods such as 1,1-diphenyl-2-
picrylhydrazyl (DPPH), 2,2-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS), nitric
oxide and hydrogen peroxide radical scavenging assays. Phytochemical constituents, total
phenolic content and total flavonoid content of the extract at different concentrations (10-500 µg/
mL) were determined.
Results: Alpinia nigra leaves showed high free radical scavenging activity as evidenced by the
low IC50 values in DPPH (64.51 µg/mL), in ABTS (28.32 µg/mL), in nitric oxide (80.02 µg/mL) and in
H2O2 (77.45 µg/mL) scavenging assays. Furthermore the TPC and TFC of the extract were found to
be 69.25 mg gallic acid equivalent per gram of extract and 78.84 mg quercetin equivalent per gram
of extract respectively.
Conclusions: The results of present comprehensive analysis demonstrated that Alpinia nigra
leaves possess high phenolic, flavonoid contents and potential antioxidant activity, and could be
used as a viable source of natural antioxidants and might be exploited for functional foods and
neutraceutical applications.
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