The individual sensitivity analysis comparing with base case was done in order to consider how and to what extent parameters of each scenario could influence the benefit gained from biofuel use. According to Fig. 4, the positive percentage presents gaining more benefit with respect to the given policy scenario. As can be seen, the results mostly correspond with the aggregated scenario set where the GHG emission impact still dominates the results. This can be seen in SC1B that shows the importance of freshwater resource used in biodiesel and bioethanol. If the weighting of this impact increases due to halving of water availability, all biofuel benefit will be lost. This is in line with what is mentioned in Damen (2010) that biomass production has a hotspot in water requirement especially to produce cassava. Meanwhile, SC2A and SC2C give less significant TEF of energy resources and GHG emissions; however, the benefits still remains but are reduced in their disadvantageous aspects. For instance, bioethanol requires a lot of energy feedstock; if the importance of this impact is reduced as given by SC2A, the overall biofuel benefit then will be reduced as well. Moreover, for SC2B, both biofuels gained benefit from weak TEF of freshwater resources. According to the results, freshwater resources related to policy has the most influence on biofuel benefit. Hence, freshwater resources’ Eco-Factor should be considered carefully in biofuel LCA study because this TEF is derived from water stress criteria, which is adjustable depending on political will and the availability of renewable water resources (rainfall can fluctuate annually) of that specific year.