Total phenolic content of the triphala extracts was determined using a modified Folin-Ciocalteu colorimetric method [28, 29]. In a 96-well microplate, 35 μL of extract was combined with 150 μL of 0.2 M Folin-Ciocalteu reagent and was allowed to react for 5 minutes. After adding 115 μL of sodium carbonate solution (7.5% w/v), the mixture was allowed to react further for 30 minutes at 45°C and finally cooled for 1 hour at room temperature. The absorbance was read at 765 nm using a microplate reader (Synergy HT, Biotek) and total phenolics were expressed as milligrams of gallic acid equivalents per gram of sample (mg GAE/g).