Highly stable colloidal solution of silver nanoparticles in a water–isopropanol–polyvinyl alcohol system was prepared through 60Co-gamma radiation at total dose of 35 kGy at dose rate of 5.67 kGy/h under nitrogen atmosphere. Ultraviolet–visible (UV–vis), X-ray diffraction (XRD), Atomic Force Microscopy (AFM) and Scanning Electron Microscopy (SEM) of the obtained colloidal solution indicated the forma- tion of spherical shaped well mono dispersed silver nanoparticles with average diameter about 30 nm having very narrow size distribution. The radiolytically obtained nanosilver colloid was coated onto cotton fabrics by a simple industrial screen printing method and its adhesion with the fabric was found out by leaching studies using Atomic Absorption Spectrophotometry (AAS). Good adhesion was achieved by the adopted method wherein 89.5% of the coated nanosilver was retained in the fabric even after keeping the fabrics soaked in water for more than 60 h. Antimicrobial efficacy tests of the nanosilver
coated cotton fabric showed that nanosilver coating is effective in killing both bacterial and fungal strains even at very low nanosilver loading (21.81 μgm/cm2). Nanosilver coating on the cotton fabric did not
allow microbes (Pseudomonas aeruginosa, Staphylococcus aureus, Escherichia coli and Candida albicans)to adhere and proliferate on fabric surface. Staphylococci (Staphylococcus aureus) and Yeast (Candida albi- cans) showed inhibition zones in presence of these nanosilver coated fabrics while no inhibition zone was observed with the uncoated control fabric.