A laboratory assembled SC-CO2 extraction apparatus (Fig. 1)
consisted of an extractor vessel (HIP Inc. Erie, PA), compression
pump (Haskel Co. Barbank, CA), pressure regulator (HIP Inc. Erie,
PA), and pressure gauge (MacDaniel Controls Co.). Twenty-five
grams of pumpkin seed powder was placed in the stainless steel
extractor with filters at the top and bottom to retain the small particles.
The liquid carbon dioxide was cooled through a glycol chiller,
compressed (using the liquid pump) and allowed to enter the
extractor at the bottom through a hot water bath (for temperature
control). The flow rate of CO2 was kept constant at 0.25 L/min for