Beach-dried green seaweed Caulerpa scalpelliformis was
procured from Central Salt and Marine Chemicals Research
Institute (CSMCRI), Mandapam Camp, Ramnad District, India.
The beach-dried seaweeds were washed with distilled water,
shade dried and stored in an airtight pack at room temperature
(28±2 ◦C). The moisture content of the dried seaweed
was 5±1% (w/w). The dye used in all the experiments was
Sandocryl golden yellow C-2G,1 a cationic dye, obtained from
Clariant India Ltd. The synthetic dye solution was prepared by
dissolving weighed amount of the dye in 1 L of distilled water.
Basic dyes fall within the class of polymethine dyes. As such
they are cationic polymethines; because ionization in solution
they possess an overall positive charge and are therefore widely
used to bind to negatively charged substrates [18]. All other
reagents used were of analytical grade. Distilled water was used
throughout the experiments.