One millimeter of sodium bicarbonate solution at a
concentration 10 times the required concentration was
transferred to glass test tubes with 9 ml of C. oleophila at
a known concentration (108 cells ml–1). The required
concentration of the sodium salt was 2%. After
incubation for 30 min at 25C, the suspension was
diluted 10-fold using 0.05 phosphate buffer (pH 7) and
plated on Petri dishes with NYDA medium (Teixido
et al., 2001). In the control water was used instead of
sodium bicarbonate. The plates were incubated at 25C
for 24 h and colonies were counted. The test was
repeated twice