greenhouse experiment
A greenhouse experiment was conducted for screening of actinomycetes strains under greenhouse condition for their antagonistic activity. Tomato nursery was grown, Four leaf stage tomato seedlings were then transplanted into new pot containing pathogen inoculum (10 -7 ml - 1 spores) cell suspension. After a week of pathogen inoculation, actinomycetes strain CPP-53 was inoculated via rootlet system at a concentration of 8 x 10 7 cfu ml -1 (seven days old culture). There were three treatments and one control, each treatment had three replicates. The experiment was repeated twice with the same actinomycetes strain. Plants were then allowed to grow under controlled greenhouse condition. Observation was made after 25 days of inoculation.