Polyclonal anti-cocaine antibody wa

Polyclonal anti-cocaine antibody was isolated from the supplied rabbit serum samples by immunoprecipitation followed by Protein A chromatography. Ammonium sulfate precipitation was performed using standard methodology [27], prior to dialysis in mini-dialysis units for 2 h against 2 L of 10 mM phosphate buffer (pH 7.4; PB). Immunochromatography was performed using Protein A spin columns with a sample capacity of