The study was designed to investigate the effects of a new ophthalmic solution containing 0.05% vitamin B12 0.05% on corneal nerve regeneration in rats after corneal injury. Eyes of anesthetized male Wistar rats were subjected to corneal injury by removing the corneal epithelium with corneal brush (Algerbrush). After the epithelial debridement, the right eye of each animal received the instillation of one drop of the ophthalmic solution containing vitamin B12 0.05% plus taurine 0.5% and sodium hyaluronate 0.5% four time per day for 10 or 30 days. Left eyes were used as control and treated with solution containing taurine 0.5% and sodium hyaluronate 0.5% alone following the same regimen. Fluorescein staining by slit-lamp and morphological analysis was used to determine corneal wound healing. Immunohistochemistry, immunoblot and confocal microscopy were used to examine corneal re-innervation. Slit-lamp and histological analyses showed that re-epithelization of the corneas was accelerated in rats treated with vitamin B12. A clear-cut difference between the two groups of rats was seen after 10 days of treatment, whereas a near-to-complete re-epithelization was observed in both groups at 30 days. Vitamin B12 treatment had also a remarkable effect on corneal re-innervation, as shown by substantial increased in the expression of neurofilament 160 and β-III tubulin at both 10 and 30 days. The presence of SV2A-positive nerve endings suggests the presence of synapse-like specialized structures in corneal epithelium of the eye treated with vitamin B12. Our findings suggest that vitamin B12 treatment represents a powerful strategy to accelerate not only re-epithelization but also corneal re-innervation after mechanical injury.
Article Outline
1. Introduction
2. Materials and methods
2.1. Chemical
2.2. Ophthalmic formulation preparation
2.3. Characteristic of ophthalmic formulation
2.3.1. pH and osmolarity
2.3.2. Rheology
2.3.3. HPLC analysis of the vitamin B12
2.4. Animals
2.5. Corneal abrasion in rats
2.6. Histological and immunohistochemical assays
2.7. Confocal microscopy
2.8. Immunoblotting
2.9. Data analysis
3. Results
3.1. Ophthalmic formulation
3.2. Determination of vitamin B12 and preliminary stability data
3.3. Effect of topical vitamin B12 on corneal epithelium after mechanic injury
3.4. Effect of topical vitamin B12 on corneal nerve regeneration
4. Discussion