At the termination of experiment, the fish were weighed 24 h after the final feeding. Then,
the fish were immersed in a solution of tricaine methanesulfonate (MS222) until euthanized.
From each tanks, fish were randomly collected, and total body weight, viscera
weight (total weight of all visceral organs), and liver weight per fish were recorded for
calculation of relative fatness (RF = (body weight - viscera weight)/body weight 9 100)
and hepatosomatic index (HSI = liver weight/body weight 9 100).