Subcultivation procedures
i) Collect aliquots of cell culture medium from all monolayers inoculated with dilutions of each
supernatant of the test homogenates.
ii) Inoculate cell monolayers as described above (Inoculation of cell monolayers, steps i and ii).
iii) Incubate and monitor as described above (Inoculation of cell monolayers, steps i and ii and
Monitoring incubation steps i and ii).