2.6. Reaction time optimization of lipase catalyzed reaction
To a mixture of waste lipid (1 g) and lipase (0.1 g, 10 wt.%),methanol (227 lL, 1:5) was added. The reaction was carried out for 36 h and the temperature was kept constant at 40 _C during the reaction. Samples were taken at 6, 12, 24 and 36 h from the reaction. All the samples were filtered to separate the enzyme. The samples were then dried under vacuum and analyzed by 1H NMR.