2×10
5
cell
suspension was seeded on each scaffold. The cell-scaffold
constructs were cultured in RPMI-l640 supplemented with
10% FBS, 1% antibiotic/antimycotic solution for up to 48h.
Finally, the cells were stained with acridine orange (AO),
which was cleaved to yield a green fluorescent product by
metabolically active cells.The density of the cells that adhered
on each scaffold was observed at 100-foldmagnification with
a fluorescence microscopy (Olympus BX51).