Fig. 5. Histological examination of ear sections of CHS-induced mice following porcine placenta extract treatment. Ears excised from each group of mouse were fixed with 4% paraformaldehyde for 16 h and embedded in paraffin. Paraffin embedded ears were sectioned into 6µm thin sections and stained with hemotoxylin (Sigma) and eosin (Sigma) (H&E). Infiltrated lymphocytes, thickening of the epidermis were observed under microscope and photographed. Black arrows indicate the increased infiltration of lymphocytes in the inflamed and thickened epidermis of the vehicle-treated group.