3. Result and Discussion
Acetobacter xylinum grown in nutrient broth medium for 24 h at room temperature were able to show good
growth (Fig. 1), then characterized by the formation of a thin layer of nata and sediment of biomass.
Fig. 1. Acetobacter xylinum in Nutrient broth medium
The calculations show the amount of biomass is ready for the immobilization of 3,5x107 CFU/mL. Then do the
immobilization process that begins with the stage of separation of biomass continued trapping process in alginate
matrix. Acetobacter xylinum trapped in the alginate matrix in the form of beads. The average number of beads
ranges between 400-450 beads/50 ml of alginate (Fig. 2).