however choice of eluent and eluent volume were
optimized for the SPE procedure for neotame. Standard neotame
solution (2 ml, 10 mg/l) was introduced into the cartridge. Acetonitrile
and methanol were used to elute neotame and it was observed
that neotame recovery was better with the use of methanol as an
eluent as compared to acetonitrile. The cartridge was washed with
10 ml of water to remove the unbound impurities; this washing
did not affect the bound neotame in the cartridge To optimize the amount of eluent needed for efficient elution,
different amounts of methanol 1, 2 and 3 ml were used to elute
the bound neotame and the eluates were analyzed for neotame
recovery. It was observed that 3 ml methanol was sufficient to
obtain a high recovery of sweetener and its degradation product