B. Media and reagents
1. Tryptose-sulfite-cycloserine (TSC) agar (M169)
2. Egg yolk emulsion, 50% (M51)
3. Chopped liver broth (M38) or cooked meat medium (modified) (M43) (chopped
liver is preferred)
4. Thioglycollate medium (fluid) (M146)
5. Iron milk medium (modified) (M68)
6. Lactose-gelatin medium (for C. perfringens) (M75)
7. Sporulation broth (for C. perfringens) (M140)
8. Motility-nitrate medium, buffered (for C. perfringens) (M102)
9. Spray's fermentation medium (for C. perfringens) (M141)
10. AE sporulation medium, modified (M5)
11. Duncan-Strong sporulation medium, modified (M45)
12. Peptone diluent (R56)
13. Nitrite detection reagents (R48)
14. Glycerin-salt solution (buffered) (R31)
15. Gram stain reagents (R32)
16. Fermentation test papers. Saturate 15 cm Whatman No. 31 filter paper disks with
0.2% aqueous bromthymol blue solution adjusted to pH 8-8.5 with ammonium
hydroxide. Air-dry the disks and store for later use.
17. Bromthymol blue, 0.04% aqueous solution (R10).