Four packages each of basil were placed in controlled-temperature chambers at 0, 5, 10,15,20, and 25C, all less than +1C, and covered
with black plastic to maintain darkness. Basil was inspected daily at 1400 HR and rated visually for the presence of necrosis, surface
molds, water soaking, chlorosis, and leaf abscission. Shelf life was defined as the time until the first obvious sign of deterioration, based on visual analysis only.