Understanding genetic structure and status of genetic variation of the Fasciola gigantica
and F. hepatica isolates from different hosts, has important implications for epidemiology and effective control of
fasciolosis. The aim of the present work was to study the molecular characterization of Fasciola gigantica and F.
hepatica isolates collected from cows and sheep, using the random amplified polymorphic DNA fragmentspolymerase
chain reaction (RAPDs-PCR) technique.F. gigantica and F. hepatica of bovine and ovine
origin were collected from the biliary tracts and gall bladders of condemned bovine and ovine livers from Cairo
Governmental slaughter house.. By using (RAPDs-PCR) technique, optimal standardization of conditions of
amplification and thermocyclation was made, using genetic markers. The methodology used compared the genetic
pattern between the two species (inter-species) and inside each species (intra-species) between cow and sheep and
the amplification fragments were between 135 and 741 base pairs of marker