4. Conclusions
A number of experiments were conducted to elucidate the impact of different parameters on the biocatalytic productivity of glucose oxidase and catalase in a membrane bioreactor. While productivity was found to depend on the oxygen availability which in turn depended on reactor configuration, hydrogen peroxide concentration and catalase origin, the contribution of other factors requires further evaluation in the particular enzyme systems that they influence. Robustness of the immobilized enzymes against local pH effects, hydrogen peroxide and other factors could possibly play a larger role than revealed in the stability studies of the free enzymes. The study contributes to explaining the differences in performance of free versus immobilized systems for glucose oxidase and catalase and other coupled enzyme reactions with similar characteristics.
Acknowledgements
This work was supported by the Bio-Value Strategic Platform for Innovation and Research, co-funded by The Danish Council for Strategic Research and The Danish Council for Technology and Innovation, Grant Case no.: 0603-00522B.