Plasmids with N gene inserts were used as external standards. RT-PCR with TSWV N-gene-specific primers (described below) yielded ≈800-bp amplicons that were used for cloning.
Plasmids with inserts N gene was used as an external standard RT-PCR with TSWV N gene-specific primers. (Described below) for amplicons ≈800 bp was used for cloning.
Plasmids with inserts were Used As external N Gene Standards. RT-PCR with TSWV N-Gene-Specific primers (described Below) yielded amplicons that were ≈800-Bp Used for cloning.