For determination of the bacillus species, the diluti on swere pretreated at 80 C for 10 min before plating on LB medium. Plates for the determination of bacteria and bacillus species were in cubated at 30 C for 36 h, for fungi and fusarium at 28 C for 72 h, and for actinomycetes at 28 C for 120 h. The results were expressedas log (CFU g -1 dry soil ).