Rajasekaran, V.Haripriya and A.Armugam Shakila their studies were conducted to standardize the type of explant and level of plant growth regulator for callus production, shoot proliferation and elongation of rhizogenesis. Different explants were used to induce callus in MS medium supplemented with Benzyl adenine (BAP) and 1AA at different concentration and reached to a conclusion that leaf bit showed highest callus ability in MS medium supplemented with 1mg 1AA /L and 10mg BAP/L within 23 days and when compared to kinetin, among the Auxin tried, 3 mg NAA/L proved to be the best for rhizogenesis (10).