Table 2
the altered proteins identified by MALDI-TOF/TOF MS in the hepatopancreas of cold-stress-treated shrimp.
Spot
number
Protein (species) Accession number Predicited
MW(kDa)/pI
Observed
MW(kDa)/pI
MASCOT
scores/match
peptides
Treated/control
Mean ± SD
NCBInr EST
Significantly down-regulated spots((expression levels were ≤0.67-fold of controls)
1 Dimethylaniline monooxygenase, putative [Cordyceps militaris
CM01]
gi|346,320,751|
EGX90351.1
62.08/6.68 33.00/5.93 NA 99/2 0.00 ± 0.00
2 Esterase D/formylglutathione hydrolase [Palaemonetes varians] gi|238,683,709|
ACR54133.1
10.31/4.64 32.00/6.04 78/1 194/3 0.48 ± 0.16
3 Chymotrypsin BI,partial[Litopenaeus vannamei] gi|2,624,387|
CAA71672.1
28.74/5.56 27.00/5.12 76/1 128/4 0.00 ± 0.00
4 Adenosine kinase 2-like [Megachile rotundata] gi|383,848,386|
XP_003699832.1
38.30/5.46 37.67/6.17 77/1 242/3 0.00 ± 0.00
5 Adenosine kinase 2-like [Megachile rotundata] gi|383,848,386|
XP_003699832.1
38.30/5.46 37.00/6.34 NA 214/3 0.00 ± 0.00
6 Putative 40S ribosomal protein S12 [Nephrops norvegicus] gi|356,483,027|CCE46016.1 15.04/5.61 16.33/5.55 137/3 209/5 0.00 ± 0.00
7 Hypothetical protein DAPPUDRAFT_311177 [Daphnia pulex] gi|321,477,837|EFX88795.1 85.22/6.49 16.33/5.84 NA 59/1 0.24 ± 0.05
8 Legumain [Rattus norvegicus] gi|56,388,620|AAH87708.1 49.48/6.09 34.00/5.07 NA 188/2 0.23 ± 0.12
9 Preamylase 1 [Litopenaeus vannamei] gi|452,675|CAA54524.1 57.00/5.21 32.33/5.23 118/2 118/2 0.00 ± 0.00
10 Legumain [Rattus norvegicus] gi|56,388,620|AAH87708.1 49.48/6.09 33.67/5.24 72/1 61/1 0.40 ± 0.15
11 Legumain [Rattus norvegicus] gi|56,388,620|AAH87708.1 49.48/6.09 33.67/5.47 NA 162/2 0.00 ± 0.00
12 Legumain-like [Oreochromis niloticus] gi|348,515,969|XP_003445512.1 48.40/5.72 33.33/5.59 NA 261/5 0.64 ± 0.07
13 Proteasome subunit alpha type-5 [Harpegnathos saltator] gi|307,209,861|EFN86640.1 26.73/4.83 30.00/5.88 86/3 320/7 0.43 ± 0.17
Significantly up-regulated spots(expression levels were ≥1.5-fold of controls)
14 Crustacyanin-A,partial
[Marsupenaeus japonicus]
gi|219,816,003|ACL37116.1 14.81/5.20 21.17/4.93 287/4 484/6 2.43 ± 0.78
15 Riboflavin-binding protein [Xenopus laevis] gi|13,021,644|AAK11502.1 21.81/5.45 18.00/5.00 NA 96/1 *
16 Dolichyl-diphosphooligosaccharide–protein glycosyltransferase
63 kDa subunit precursor, putative [Pediculus humanus corporis]
gi|242,025,614|XP_002433219.1 68.24/9.21 40.50/6.09 NA 240/4 2.71 ± 0.86
17 Actin 1 [Penaeus monodon] gi|3,907,620|AAC78681.1 41.74/5.23 45.33/5.36 301/4 222/3 *
18 Hemocyanin [Litopenaeus vannamei] gi|854,403|CAA57880.1 74.98/5.27 44.33/5.08 163/4 141/3 *
19 Hemocyanin [Litopenaeus vannamei] gi|854,403|CAA57880.1 74.98/5.27 44.33/5.17 74/1 74/1 *
20 Proteinase inhibitor I4, serpin [Beggiatoa sp. PS] gi|153,872,807|ZP_02001593.1 43.40/7.85 42.33/5.22 54/1 70/3 7.46 ± 0.79
21 ABC transporter, ATP-binding protein [Streptococcus sp. oral
taxon 058 str. F0407]
gi|358,464,297|ZP_09174262.1 65.35/5.69 42.33/5.11 NA 88/3 8.34 ± 0.97
22 Fructose 1,6-bisphosphatase [Marsupenaeus japonicus] gi|310,743,900|BAJ23881.1 36.64/6.45 29.00/5.96 NA 75/1 *
23 Ankyrin repeat domain-containing protein 13C [Salmo salar] gi|221,221,352|ACM09337.1 16.08/9.07 31.00/6.04 NA 65/1 *
24 Chitinase [Litopenaeus vannamei] gi|25,229,108|AAN74647.1 51.96/4.87 34.50/5.27 402/6 220/3 2.21 ± 0.55
25 NADH:ubiquinone dehydrogenase, putative [Aedes aegypti]
(similar to NADH Dehydrogenase (Ubiquinone) 1 Alpha
Subcomplex 8, mRNA sequence)
gi|157,107,772|
XP_001649930.1
19.90/6.44 23.00/6.12 NA 80/1 *
26 Thioredoxin-dependent peroxide reductase [Ixodes scapularis] gi|67,083,335|
AAY66603.1
25.69/9.10 20.00/5.48 95/1 134/2 *
27 Malic enzyme [Aedes aegypti] gi|157,114,273|
XP_001658019.1
72.03/7.22 32.67/6.45 82/2 119/3 *
28 Chitinase [Litopenaeus vannamei] gi|25,229,108|AAN74647.1 51.96/4.87 34.67/5.43 92/3 92/1 *
29 3′(2′),5′-bisphosphate nucleotidase 1-like isoform 1 [Nasonia
vitripennis]
gi|156,551,199|XP_001604882.1 32.98/5.60 39.67/5.07 NA 112/2 *
30 Crustacyanin-A,partial
[Marsupenaeus japonicus]
gi|219,816,003|
ACL37116.1
14.81/5.20 32.00/6.47 181/2 476/5 4.56 ± 0.79
31 Fructose 1,6-bisphosphatase [Marsupenaeus japonicus] gi|310,743,900|BAJ23881.1 36.64/6.45 29.00/6.32 118/4 177/2 *
32 Glutathione S-transferase Mu 3-like [Oreochromis niloticus] gi|348,514,674|XP_003444865.1 25.72/5.82 15.00/5.31 NA 277/5 *
33 Xanthine dehydrogenase [Crassostrea gigas] gi|405,967,948|EKC33062.1 135.93/5.88 28.00/6.05 NA 211/5 1.89 ± 0.34
34 Crustacyanin-A,partial
[Marsupenaeus japonicus]
gi|219,816,003|
ACL37116.1
14.81/5.20 23.00/5.22 145/5 414/5 1.87 ± 0.08
35 Glucose-regulated protein 78 [Fenneropenaeus chinensis] gi|124,245,114|
ABM92447.1
72.73/5.00 29.00/5.08 132/2 157/2 1.67 ± 0.28
36 Putative regucalcin (senescence marker protein-30) [Pipa
carvalhoi]
gi|349,803,671|
AEQ17308.1
31.80/5.08 39.33/5.49 NA 61/1 6.81 ± 2.01
37 Ribulose-5-phosphate 4-epimerase-like protein [Branchio
Table 2the altered proteins identified by MALDI-TOF/TOF MS in the hepatopancreas of cold-stress-treated shrimp.SpotnumberProtein (species) Accession number PredicitedMW(kDa)/pIObservedMW(kDa)/pIMASCOTscores/matchpeptidesTreated/controlMean ± SDNCBInr ESTSignificantly down-regulated spots((expression levels were ≤0.67-fold of controls)1 Dimethylaniline monooxygenase, putative [Cordyceps militarisCM01]gi|346,320,751|EGX90351.162.08/6.68 33.00/5.93 NA 99/2 0.00 ± 0.002 Esterase D/formylglutathione hydrolase [Palaemonetes varians] gi|238,683,709|ACR54133.110.31/4.64 32.00/6.04 78/1 194/3 0.48 ± 0.163 Chymotrypsin BI,partial[Litopenaeus vannamei] gi|2,624,387|CAA71672.128.74/5.56 27.00/5.12 76/1 128/4 0.00 ± 0.004 Adenosine kinase 2-like [Megachile rotundata] gi|383,848,386|XP_003699832.138.30/5.46 37.67/6.17 77/1 242/3 0.00 ± 0.005 Adenosine kinase 2-like [Megachile rotundata] gi|383,848,386|XP_003699832.138.30/5.46 37.00/6.34 NA 214/3 0.00 ± 0.006 Putative 40S ribosomal protein S12 [Nephrops norvegicus] gi|356,483,027|CCE46016.1 15.04/5.61 16.33/5.55 137/3 209/5 0.00 ± 0.007 Hypothetical protein DAPPUDRAFT_311177 [Daphnia pulex] gi|321,477,837|EFX88795.1 85.22/6.49 16.33/5.84 NA 59/1 0.24 ± 0.058 Legumain [Rattus norvegicus] gi|56,388,620|AAH87708.1 49.48/6.09 34.00/5.07 NA 188/2 0.23 ± 0.129 Preamylase 1 [Litopenaeus vannamei] gi|452,675|CAA54524.1 57.00/5.21 32.33/5.23 118/2 118/2 0.00 ± 0.0010 Legumain [Rattus norvegicus] gi|56,388,620|AAH87708.1 49.48/6.09 33.67/5.24 72/1 61/1 0.40 ± 0.15
11 Legumain [Rattus norvegicus] gi|56,388,620|AAH87708.1 49.48/6.09 33.67/5.47 NA 162/2 0.00 ± 0.00
12 Legumain-like [Oreochromis niloticus] gi|348,515,969|XP_003445512.1 48.40/5.72 33.33/5.59 NA 261/5 0.64 ± 0.07
13 Proteasome subunit alpha type-5 [Harpegnathos saltator] gi|307,209,861|EFN86640.1 26.73/4.83 30.00/5.88 86/3 320/7 0.43 ± 0.17
Significantly up-regulated spots(expression levels were ≥1.5-fold of controls)
14 Crustacyanin-A,partial
[Marsupenaeus japonicus]
gi|219,816,003|ACL37116.1 14.81/5.20 21.17/4.93 287/4 484/6 2.43 ± 0.78
15 Riboflavin-binding protein [Xenopus laevis] gi|13,021,644|AAK11502.1 21.81/5.45 18.00/5.00 NA 96/1 *
16 Dolichyl-diphosphooligosaccharide–protein glycosyltransferase
63 kDa subunit precursor, putative [Pediculus humanus corporis]
gi|242,025,614|XP_002433219.1 68.24/9.21 40.50/6.09 NA 240/4 2.71 ± 0.86
17 Actin 1 [Penaeus monodon] gi|3,907,620|AAC78681.1 41.74/5.23 45.33/5.36 301/4 222/3 *
18 Hemocyanin [Litopenaeus vannamei] gi|854,403|CAA57880.1 74.98/5.27 44.33/5.08 163/4 141/3 *
19 Hemocyanin [Litopenaeus vannamei] gi|854,403|CAA57880.1 74.98/5.27 44.33/5.17 74/1 74/1 *
20 Proteinase inhibitor I4, serpin [Beggiatoa sp. PS] gi|153,872,807|ZP_02001593.1 43.40/7.85 42.33/5.22 54/1 70/3 7.46 ± 0.79
21 ABC transporter, ATP-binding protein [Streptococcus sp. oral
taxon 058 str. F0407]
gi|358,464,297|ZP_09174262.1 65.35/5.69 42.33/5.11 NA 88/3 8.34 ± 0.97
22 Fructose 1,6-bisphosphatase [Marsupenaeus japonicus] gi|310,743,900|BAJ23881.1 36.64/6.45 29.00/5.96 NA 75/1 *
23 Ankyrin repeat domain-containing protein 13C [Salmo salar] gi|221,221,352|ACM09337.1 16.08/9.07 31.00/6.04 NA 65/1 *
24 Chitinase [Litopenaeus vannamei] gi|25,229,108|AAN74647.1 51.96/4.87 34.50/5.27 402/6 220/3 2.21 ± 0.55
25 NADH:ubiquinone dehydrogenase, putative [Aedes aegypti]
(similar to NADH Dehydrogenase (Ubiquinone) 1 Alpha
Subcomplex 8, mRNA sequence)
gi|157,107,772|
XP_001649930.1
19.90/6.44 23.00/6.12 NA 80/1 *
26 Thioredoxin-dependent peroxide reductase [Ixodes scapularis] gi|67,083,335|
AAY66603.1
25.69/9.10 20.00/5.48 95/1 134/2 *
27 Malic enzyme [Aedes aegypti] gi|157,114,273|
XP_001658019.1
72.03/7.22 32.67/6.45 82/2 119/3 *
28 Chitinase [Litopenaeus vannamei] gi|25,229,108|AAN74647.1 51.96/4.87 34.67/5.43 92/3 92/1 *
29 3′(2′),5′-bisphosphate nucleotidase 1-like isoform 1 [Nasonia
vitripennis]
gi|156,551,199|XP_001604882.1 32.98/5.60 39.67/5.07 NA 112/2 *
30 Crustacyanin-A,partial
[Marsupenaeus japonicus]
gi|219,816,003|
ACL37116.1
14.81/5.20 32.00/6.47 181/2 476/5 4.56 ± 0.79
31 Fructose 1,6-bisphosphatase [Marsupenaeus japonicus] gi|310,743,900|BAJ23881.1 36.64/6.45 29.00/6.32 118/4 177/2 *
32 Glutathione S-transferase Mu 3-like [Oreochromis niloticus] gi|348,514,674|XP_003444865.1 25.72/5.82 15.00/5.31 NA 277/5 *
33 Xanthine dehydrogenase [Crassostrea gigas] gi|405,967,948|EKC33062.1 135.93/5.88 28.00/6.05 NA 211/5 1.89 ± 0.34
34 Crustacyanin-A,partial
[Marsupenaeus japonicus]
gi|219,816,003|
ACL37116.1
14.81/5.20 23.00/5.22 145/5 414/5 1.87 ± 0.08
35 Glucose-regulated protein 78 [Fenneropenaeus chinensis] gi|124,245,114|
ABM92447.1
72.73/5.00 29.00/5.08 132/2 157/2 1.67 ± 0.28
36 Putative regucalcin (senescence marker protein-30) [Pipa
carvalhoi]
gi|349,803,671|
AEQ17308.1
31.80/5.08 39.33/5.49 NA 61/1 6.81 ± 2.01
37 Ribulose-5-phosphate 4-epimerase-like protein [Branchio
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