Pumps, respectively A (eluent A) and B (eluentB) were programmed according to the following sequence (values in %), 0–14 min: 67:33, 17 min: 75:25, 26 min: 73:27, 27 min:
67:33. Flow rate was 1.5 mL/min, injection volume 50L and run time 30min (except for orange, which was 105 min).