Cell counting is an important routine procedure. However, to date there is no comprehensive, easy to use,
and inexpensive solution for routine cell counting, and this procedure usually needs to be performed
manually. Here, we report a complete solution for automatic cell counting in which a conventional light
microscope is equipped with a web camera to obtain images of a suspension of mammalian cells in a
hemocytometer assembly. Based on the ImageJ toolbox, we devised two algorithms to automatically
count these cells. This approach is approximately 10 times faster and yields more reliable and consistent
results compared with manual counting.