2.8. Statistical analysis
All the greenhouse experiments were arranged in completely
randomized block design with six replications. Field experiment
was conducted in a completely randomized block design with three
replications. Dual-culture assay was conducted in completely
randomized design with three replications [For FOC: 411 Petri
plates were used (137 actinomycetes 3 replications); for
M. phaseolina (1 strain) and R. bataticola (3 strains): 60 plates were
used (5 actinomycetes 3 replications)]. The data were subjected
to analysis of variance (ANOVA) (GenStat 10.1 version 2007, Lawes
Agricultural Trust, Rothamsted Experimental Station) to evaluate
the efficiency of biocontrol agent’s application in the greenhouse
studies. Significance of differences between the treatment means
was tested at P ¼ 0.01 and 0.05.