40 ll paraffin oil was added and after denaturation firstly occurs for 5 min at 96 C, followed by 35 PCR cycles that consist of denaturation at 95 C/for 3 min, annealing at 55 C/45 s, extension at 72 C/45 s, and final extension at 72 C/7 min.
40 ll paraffinoil was added and after denaturation firstly occurs for 5 min at96 C, followed by 35 PCR cycles that consist of denaturationat 95 C/for 3 min, annealing at 55 C/45 s, extension at72 C/45 s, and final extension at 72 C/7 min.