Each inoculum was prepared by inoculating the slant culture into 25 mL of the sterile liquid medium
in 100 mL conical flask and growing it on a rotary shaker (100 rpm) for 48 h. 20% (v/v) inoculums (6 x
104 cell/mL) was inoculated into 100 mL sterile pineapple peel hydrolysate in 250 mL conical flask and
was incubated up to 5 days under stationary conditions. All the state experiments were conducted at pH
5.0 and 30oC [1].