found that whey that could be expressed by centrifugation was
somehow associated with fat globules. As observed by confocal
microscopy, homogenisation causes the fat globules to become part of
the para-casein network through the incorporation of micellar casein
in the surface coat of fat globules. After centrifugation, a layer of fat
could be observed at the top of the supernatant from PA-cheeses. The
release of fat in PA-cheeses, which was filling voids of the protein
matrix, would allow a higher packing of the pellet, resulting in higher
water release compared to cheeses from homogenised milks.