Characterization Morphological characterizations of chitin NFs, chitosan NPs, chi-tosan NFs and water soluble chitosan oligomers were performedusing Atomic Force Microscope (AFM) using a Park Systems, XE-100 microscope. Imaging was carried out using non-contact modecantilever, having a tip radius less than 10 nm operating at a scan-ning frequency of 0.5 Hz. Chitin NFs was first dispersed in waterusing ultrasound tip. Then drop of the solution was casted onto amica piece which is later mounted on the scanning stage of AFMafter complete drying.Transmission electron microscopy (TEM) was used to character-ize the morphology of the prepared samples using JEOL JEM-1011operating at an accelerating voltage of 100 kV. Water dispersionsof the nanomaterials were casted on TEM grids and allowed fordrying. Dried grids were taken for inspection.Fourier transform infrared spectroscopy (FTIR) measurementswere taken for chitin flakes, chitin NFs and for the mixture of chi-tosan NFs and chitosan NPs (Bruker FT-IR Vertex 80). Dried sampleswere placed on top of the ZnSe crystal inside the ATR chamber