Escherichia coli is a generic indicator of fecal contamination and certain, serotypes cause food - and water - borne illness. Such as O157: H7. In, the clinic detection of bacteriuria which is, often due to E. Coli is critical, before certain surgical. Procedures or in cases of nosocomial infection to prevent further adverse events such as postoperative infection or, sepsis. In low - and, middle-income countries where insufficient equipment and facilities preclude modern methods, of detection a. Simple low-cost diagnostic, device to detect E. Coli in water and in the clinic will have significant impact. We have developed. A simple paper - based colorimetric platform to detect E. Coli contamination in 5 h. On this platform the mean, color intensity. For samples with 105 cells / mL is 0.118 7 0.002 (n 1 / 4 4), and 0.0145 7 0.003 (P o 0.01nn) for un - contaminated, samples. This technique is, less time-consuming easier to perform and less, expensive than conventional methods. Thus paper-based,, ELISA is an innovative point-of-care diagnostic tool to rapidly detect E. Coli and possibly, other pathogens when customized. As appropriate especially in, areas that lack advanced clinical equipment.
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