organisms that growth aerobically are routinely exposed to oxidative stress in the form of reactive oxygen species.
Monitoring the dynamic variations of oxidative stess allows us to understand its role in basic cellular function and determine mechanisms of antioxidant.
In this study, microdialysis sampling was employed for continuous monitoring of the formation of malondialdehyde in a bacterium-inoculated broth.
To test the practicality of this approach, oxidative stress was induced by cadmium and then a 60-min interval was selected to collect sufficient amounts of dialysate for HPLC with fluorescence detection.
After optimization of this simple-to-operate, simultaneous, and continuous method for dynamic monitoring of MDA durind periods of bacterial growth, a retrodialysis technique and a no-net-flux method were used to assess the probe recovery and analytical performance of the proposed system.