phytase activities were calculated for both extra- and intracellular
phytases. The activity was measured at four different time points (0,
15, 30 and 45 min) and ΔCPO4 was calculated (Olstorpe et al., 2009).
Enzyme activity was expressed as milliUnit per ml of enzyme extract
(mU/ml). All assays were performed in triplicates and the mean and
standard errors of the mean calculated.
2.5. Statistics
The significance of the variations was determined by ANOVA
(XLStat, Addinsoft SARL, Paris, France). Tukey's honestly significant difference
post-hoc comparisonwas performed and a p value of b0.05 was
considered significant. Correlation coefficients were calculated by using
Pearson correlation