The Connectivity Analysis Toolkit provides conservation planners with newly-developed tools for both linkage mapping and landscape-level 'centrality' analysis. Centrality refers to a group of landscape metrics that rank the importance of sites as gatekeepers for flow across a landscape network. The Toolkit allows users to develop and compare three contrasting centrality metrics based on input data representing habitat suitability or permeability, in order to determine which areas, across the landscape as a whole, would be priorities for conservation measures that might facilitate connectivity and dispersal. The Toolkit also allows application of these approaches to the more common question of mapping the best habitat linkages between a source and a target patch.