Appropriate amounts of poloxamer 407 were slowly added
to cold distilled water at 5 C to yield 20% and 30% gels and constant
stirring was maintained [17]. The poloxamer solution was
kept refrigerated until a clear solution was obtained (6–12 h). For
the gel to be formed, the solution should be kept at 30 C. The
appropriate amounts of ALA or ALA cubosome dispersions were
then added to the gels to yield 5%.