Segments of samples, held in a dorsol-ventral position,
The fixation tissue samples were washed with phosphate buffer for 10 min, and this washing procedure was applied twice
Post fixation was continued with 2% Osmic acid at 4 8C for 24 h.
Tissue samples were washed with 0.1 M phosphate buffer (pH 7.4) during 3 h with changes every 15 min.