To date, tropical orchids appear to commonly associate with mycorrhizal fungi assignable to Tulasnella
(Basidiomycota) for their seed germination and developmental needs in situ. In this study, two Tulasnella
strains (JC-02 and JC-05) isolated from roots of an endangered species, Dendrobium nobile Lindl (Orchidaceae)
collected from Yunnan province in China, were identified using the nuclear ribosomal internal
transcribed spacer (ITS) and 5.8S rDNA sequences. Seed germination and plant growth were evaluated
up to 11 weeks and 7 weeks after interaction with Tulasnella fungi, respectively. The results revealed
that the two isolates could promote seeds germination up to stage 5 after sowing for 11 weeks, and the
rates of germination were 98.47% and 99.05%, respectively, higher than that of control (81.05%). Without
fungi, seed development was arrested at stage 2. After inoculating Tulasnella isolate to seedlings for 49
days, it was found that mycelium formed pelotons in the cortical cells of roots in the form of intact and
degenerate pelotons. Significant differences were detected between the control group and the experimental
group treated with Tulasnella isolates in dry weight and fresh weight of plants respectively.
The study firstly demonstrated that two different strains of Tulasnella vary in their ability to facilitate
germination/development of D. officinale in vitro.
To date, tropical orchids appear to commonly associate with mycorrhizal fungi assignable to Tulasnella(Basidiomycota) for their seed germination and developmental needs in situ. In this study, two Tulasnellastrains (JC-02 and JC-05) isolated from roots of an endangered species, Dendrobium nobile Lindl (Orchidaceae)collected from Yunnan province in China, were identified using the nuclear ribosomal internaltranscribed spacer (ITS) and 5.8S rDNA sequences. Seed germination and plant growth were evaluatedup to 11 weeks and 7 weeks after interaction with Tulasnella fungi, respectively. The results revealedthat the two isolates could promote seeds germination up to stage 5 after sowing for 11 weeks, and therates of germination were 98.47% and 99.05%, respectively, higher than that of control (81.05%). Withoutfungi, seed development was arrested at stage 2. After inoculating Tulasnella isolate to seedlings for 49days, it was found that mycelium formed pelotons in the cortical cells of roots in the form of intact anddegenerate pelotons. Significant differences were detected between the control group and the experimentalgroup treated with Tulasnella isolates in dry weight and fresh weight of plants respectively.The study firstly demonstrated that two different strains of Tulasnella vary in their ability to facilitategermination/development of D. officinale in vitro.
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