Combined effects of treatments employing UV-C radiation followed
by various antimicrobial wash for inactivation of S. enterica
on plum tomato surface are presented in Table 1. The recovered
initial population (mean value) of Salmonella from tomato was
7.7 ± 0.6 log CFU per tomato fruit. In the present work, a control
water wash (2 min) removed 0.76 ± 0.40 log CFU/fruit; and UV-C
treatment (0.6 kJ/m2) alone inactivated 1.6 ± 0.31 log CFU/fruit
respectively. According to the table (Table 1), all four organic acids
(AA, LA, CA, and MA) and their binary formulations indicated strong
inactivation with UV-C. Organic acids were chosen in combination
treatment since they are capable of preventing bacterial growth
effectively through an entirely different mechanism than UV-C and
since these acids are GRAS, economic, and easy to manipulate.
Bactericidal effect of organic acids is due to acidification of cytoplasm
with consequential uncoupling of energy source and accumulation
of dissociated acid anion to toxic levels (Taylor et al.,
2012). A preliminary experiment was conducted to evaluate the
efficacy of individual acids by immersing an inoculated tomato in
0.5%,1% and 2% solutions for 1e4 min. As shown in Fig.1,we did not
observe any significant difference in bactericidal effects for these
acids above the concentration level of 1%, beyond 2 min. Therefore,
2 min wash in a 1% solution was chosen as the optimum in all
hurdle experiments involving organic acids or their binary combinations.
As given in Table 1, the combined effect of 1% AA and UV-C
(3.42 ± 0.4 log CFU/fruit log reduction) was lower compared to
other hurdles involving organic acids. Among the tested organic
acids, the binary mixture of 1% LA & CA in combination with UV-C
showed best inactivation efficacy (4.69 ± 0.59 log CFU/fruit) which
Combined effects of treatments employing UV-C radiation followedby various antimicrobial wash for inactivation of S. entericaon plum tomato surface are presented in Table 1. The recoveredinitial population (mean value) of Salmonella from tomato was7.7 ± 0.6 log CFU per tomato fruit. In the present work, a controlwater wash (2 min) removed 0.76 ± 0.40 log CFU/fruit; and UV-Ctreatment (0.6 kJ/m2) alone inactivated 1.6 ± 0.31 log CFU/fruitrespectively. According to the table (Table 1), all four organic acids(AA, LA, CA, and MA) and their binary formulations indicated stronginactivation with UV-C. Organic acids were chosen in combinationtreatment since they are capable of preventing bacterial growtheffectively through an entirely different mechanism than UV-C andsince these acids are GRAS, economic, and easy to manipulate.Bactericidal effect of organic acids is due to acidification of cytoplasmwith consequential uncoupling of energy source and accumulationof dissociated acid anion to toxic levels (Taylor et al.,2012). A preliminary experiment was conducted to evaluate theefficacy of individual acids by immersing an inoculated tomato in0.5%,1% and 2% solutions for 1e4 min. As shown in Fig.1,we did notobserve any significant difference in bactericidal effects for theseacids above the concentration level of 1%, beyond 2 min. Therefore,2 min wash in a 1% solution was chosen as the optimum in allรั้วกระโดดข้ามทดลองที่เกี่ยวข้องกับกรดอินทรีย์หรือชุดของไบนารีที่กำหนดในตารางที่ 1 ผลรวมของ 1% AA และ UV-C(3.42 ± 0.4 ล็อก CFU/ผลไม้ ลดล็อก) ต่ำกว่าเมื่อเทียบกับอุปสรรคอื่น ๆ ที่เกี่ยวข้องกับกรดอินทรีย์ ระหว่างการทดสอบอินทรีย์กรด ผสมไบนารี 1% LA และ CA ร่วมกับ UV-Cแสดงให้เห็นว่าประสิทธิภาพดีที่สุดในยกเลิกการเรียก (4.69 ±คือ 0.59 ล็อก CFU/ผลไม้) ซึ่ง
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