Materials and method
2.1. Beef samples
Beef Semitendinosus (ST) muscle from Limousin cross heifers were
obtained at 48 h post mortem from local meat supplier (Kepak,
Clonee, Co. Dublin, Ireland). The muscle was stored under refrigeration
overnight. After 72 h post mortem, the muscle was trimmed of
all visible fat and cut into strips (6×2×2 cm, ~30 g) parallel to the
muscle fibre direction using a guided chopping board. Samples were
randomly assigned to treatments. Three different ST muscles were
analysed.