Cells were placed in a 25 ml T-flask at a density of 1105 cells/flask in Dulbecco’s Modified Eagle’s Medium (DMEM, Sigma) containing 4.5 g/l of glucose, 10% (v/v) fetal bovine serum (FBS), and 1% (v/v) antibiotic-antimycotic (Gibco, Auckland, N.Z.)
Cells were placed in a 25 ml T-flask at a densityof 1105 cells/flask in Dulbecco’s Modified Eagle’sMedium (DMEM, Sigma) containing 4.5 g/l of glucose, 10%(v/v) fetal bovine serum (FBS), and 1% (v/v) antibiotic-antimycotic(Gibco, Auckland, N.Z.)