Optimal conditions obtained for SP hydrolysis inliquefaction step by -amylase from previous experimentwas applied to the prepared SP mash. Then, liquefiedproduct was added with following components: 10.0 g/Lbacteriological peptone, 8.0 g/L meat extract, 4.0 g/L yeastextract, 1.0 ml/L Tween 80, 2.0 g/L (NH4)3C6H5O7, 5.0 g/LCH3COONa,