After 30 days, 15 samples (93.75%) showed genomic expression in group 1, 9 samples (60%) showed genomic expression in group 2, and 8 samples (57%) showed genomic expression in group 3. Five samples in group 1, 11samples in group 2, and 12 samples in group 3 were genetically indeterminate (Table I) (calculated by cycle threshold values greater than 40). In group 1, 18% (3 of 16) of the samples showed a reduction in S mutans concentration, whereas in groups 2 and 3, all samples (15 of 15, and 14 of 14, respectively) showed reductions in S mutans concentrations (calculated by the increase in cycle threshold values). The samples with increases in the cycle threshold values after 30 days would have reductions in S mutans