The preparation of bacterial inocula was done by using
18 h old overnight bacterial cultures prepared in Nutrient Agar. A few
colonies of bacteria were collected aseptically with a sterile loop and
introduced into 10 ml of sterile 0.90% saline solution. The concentration
of the suspension was then standardized by adjusting the optical density
to 0.10 at 630 nm, corresponding to bacterial cell suspension of 108
colony-forming units/mL (CFU/mL) [17]